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BioSequenceReverseComplement[bioseq]

biologically complements and reverses the sequence bioseq.

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Basic Examples  
Generalizations & Extensions  
Properties & Relations  
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BioSequenceReverseComplement

BioSequenceReverseComplement[bioseq]

biologically complements and reverses the sequence bioseq.

Details

  • The complement of a degenerate letter, which represents one of a number of potential bases, is another degenerate letter representing all potential complement bases.
  • The reverse complement specifies the genetic sequence as read from a complement strand.

Examples

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Basic Examples  (1)

Obtain the reverse complement sequences for both DNA and RNA sequences:

Wolfram Language code: BioSequenceReverseComplement[BioSequence["DNA", "AGG"]]
Wolfram Language code: BioSequenceReverseComplement[BioSequence["RNA", "AGG"]]

Generalizations & Extensions  (3)

Reverse complements of DNA and RNA sequences also apply to sequences with degenerate letters:

Wolfram Language code: BioSequenceReverseComplement[BioSequence["DNA", "WWB"]]
Wolfram Language code: BioSequenceReverseComplement[BioSequence["RNA", "WWB"]]

Reverse complements can also be taken for circular DNA:

Wolfram Language code: BioSequenceReverseComplement[BioSequence["CircularDNA", "AGG"]]

Reverse complements can be applied to extended types with the "ComplementLetterRules" property:

Wolfram Language code: es = EntityStore["ExtendedBioSequenceType" -> <| "Entities" -> <| "HachimojiDNA" -> <| "Alphabet" -> {"C", "G", "T", "A", "B", "P", "S", "Z"}, "Caption" -> "Hachimoji DNA Sequence", "Icon" -> "DNA", "Source" -> "DOI: 10.1126/science.aat0971", "ComplementLetterRules" -> {"A"  "T", "C"  "G", "P"  "Z", "B"  "S"} |> |>|>]; EntityRegister[es]; example = BioSequence["HachimojiDNA", "BSCGGZ"]
Wolfram Language code: BioSequenceReverseComplement[example]

Properties & Relations  (1)

BioSequenceReverseComplement is its own inverse:

Wolfram Language code: BioSequenceReverseComplement[BioSequenceReverseComplement[BioSequence["DNA", "AGG"]]]

Possible Issues  (1)

Reverse complementation is not a biological operation that applies to peptide sequences:

Wolfram Language code: BioSequenceReverseComplement[BioSequence["Peptide", "LFF"]]
Wolfram Research (2020), BioSequenceReverseComplement, Wolfram Language function, https://reference.wolfram.com/language/ref/BioSequenceReverseComplement.html.

Text

Wolfram Research (2020), BioSequenceReverseComplement, Wolfram Language function, https://reference.wolfram.com/language/ref/BioSequenceReverseComplement.html.

CMS

Wolfram Language. 2020. "BioSequenceReverseComplement." Wolfram Language & System Documentation Center. Wolfram Research. https://reference.wolfram.com/language/ref/BioSequenceReverseComplement.html.

APA

Wolfram Language. (2020). BioSequenceReverseComplement. Wolfram Language & System Documentation Center. Retrieved from https://reference.wolfram.com/language/ref/BioSequenceReverseComplement.html

BibTeX

@misc{reference.wolfram_2026_biosequencereversecomplement, author="Wolfram Research", title="{BioSequenceReverseComplement}", year="2020", howpublished="\url{https://reference.wolfram.com/language/ref/BioSequenceReverseComplement.html}", note=[Accessed: 13-June-2026]}

BibLaTeX

@online{reference.wolfram_2026_biosequencereversecomplement, organization={Wolfram Research}, title={BioSequenceReverseComplement}, year={2020}, url={https://reference.wolfram.com/language/ref/BioSequenceReverseComplement.html}, note=[Accessed: 13-June-2026]}